AttoPhos
AttoPhos and AttoPhos Plus are novel alkaline phosphatase substrates invented by JBL Scientific Inc. and distributed by Promega. They can be used in any assay where alkaline phosphatase is the amplification molecule such as Western blots and ELISAs. The excitation and emission maxima of AttoPhos are 440 nm and 560 nm respectively - ideal for use with the Dark Reader. In conjunction with the Dark Reader, AttoPhos can be used to detect less than 100 pg of target protein.
western blot stained with attophos A Western blot of a standard protein set at various dilutions and stained with AttoPhos Plus (kindly provided by JBL Scientific) was viewed on either a Dark Reader transilluminator or a 312 nm UV device. The DR is about 2 times more sensitive.
Clare Chemical Research

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