No UV Output: No DNA Damage.

DNA undergoes a number of reactions when exposed to UV light. Because the Dark Reader does not emit any UV light, the extent of DNA damage is drastically reduced compared to the damage produced by a UV device.
No DNA nicking with a Dark Reader
100 ng of supercoiled (sc) plasmid was placed on either a Dark Reader (DR) or a 312 nm UV transilluminator (UV) for various times. The DNA was then digested with T4 endonuclease V, which excises T:T dimers, and run on an agarose gel and viewed.

It is clear that as little as a 5 sec exposure to UV light is sufficient to convert almost 100% of the plasmid into the relaxed (rx) form and after 300 sec the DNA is completely fragmented. In contrast, a 300 sec exposure on the Dark Reader resulted in no detectable damage to the plasmid.
Clare Chemical Research

FAQ
User Feedback
Dark Reader Lit.
Journal Refs.
Links
SiteMap
No UV
No DNA Damage
Improve Cloning
Cut Bleaching
See through Walls
Contact
Transilluminators
Lamps
GelHead
Accessories
Home
Theory
Ordering
GelGreen
SYBR Gold
SYBR Green
SYBR Safe
GelStar
Ethidium
GFPs
Transgenics
fluorescein
Pro-Q Diamond
SYPRO Ruby
SYPRO Orange
How it Works
Which Dyes?
Products
Support
Examples