PBXL
PBXL fluorophors (Martek Biosciences Corporation)are based around light-harvesting proteins isolated from cyanobacteria and red algae. They deliver up to 1400 chromophores per binding event without signal processing, signal amplification or enzyme substrates. Available as a variety of conjugates with either streptavidin, or IgG, PBXL dyes can be used in numerous blotting and immunoassay applications that previously required more tedious enzyme amplification methods.
PBXL detection with a Dark Reader PBXL-1 directly spotted onto a nitrocellulose membrane (kindly provided by Dr. Thomas Allnutt, Martek Biosciences) was imaged using a DR45 transilluminator and a basic CCD camera system. The results of the digital analysis are plotted on the right.

It was possible to detect attomolar levels of the dye - even though the DR has not yet been specifically optimized for use with PBXL-1 (ex. max = 545 nm, em. max = 666 nm).
Clare Chemical Research

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