Group Leader, Bioanalytical Assay Development
Molecular Probes, Inc.
The dye is available in 3 forms:
- SYPRO Ruby Protein Gel stain allows sensitive fluorescence detection of proteins on SDS-polyacryamide, and two-dimensional gels.
- SYPRO Ruby IEF gel stain detects proteins in carrier ampholyte isoelectric focusing, immobilized pH and nondenaturing gels.
- SYPRO Ruby Protein Blot stain is appropriate for high sensitivity detection of proteins on PVDF and nitrocellulose membranes.
About 2 ng of SYPRO Ruby-stained protein can be detected directly by eye in an SDS gel using the Dark Reader and about 8 ng on a blot.
Both contain 12 lanes of 2-fold serial dilutions of Bio-Rad mid-range molecular weight markers, with the highest concentration corresponding to 2000 ng per protein band.
Both were stained with SYPRO Ruby and viewed using a Dark Reader transilluminator.
