See through Walls !

Maybe not brick walls; but certainly test-tube walls, 96-well plate walls, gel plate walls..... all sorts of plastic and glass walls.

There are 2 reasons for the superiority of the Dark Reader compared to UV:
    1. No Blocked Light
    EGFP as it migrates down a native protein gel
    Dark Reader blue light easily passes through transparent materials whereas UV is often blocked or severely attenuated. Consequently, the Dark Reader will be much more effective at exciting fluorophors.
    An example of how to block UV: the gel apparatus consists of several layers - the protective outer cover, the buffer reservoir and the electrophoresis plates. Using UV light, absolutely no sample fluorescence is visible. With a Dark Reader Hand Lamp, the EGFP can be clearly seen migrating down the gel.
    2. No Background
    Fluorescein in tubes
    Many plastics fluoresce when exposed to UV light. The result is a high background fluorescence that masks any fluorescence from the actual sample.
    An example of background: Fluorescein in a 1.5 mL plastic tube was viewed using either a Dark Reader Hand Lamp or a UV device. In the case of UV irradiation, the fluorescence from the sample is difficult to separate from the fluorescence from the tube itself. Using a Dark Reader, there is essentially no fluorescence from the tube and the fluorescein sample is clearly visible.
    The Typical Research Lab
    The typical research lab is crammed with apparatus, containers and other devices made from glass or plastics that may severely degrade the usefulness of UV light to detect fluorophors. The data presented in the graph below show that using a Dark Reader instead, results in an 8- to 16-fold improvement in the sensitivity of detection.
    The Dark Reader out-performs UV by a factor of 8 - 16.
    The effectiveness of the Dark Reader for viewing fluorescein.
    A 2-fold dilution series of fluorescein was variously aliquoted onto assorted laboratory-ware. The dilution series was then viewed by eye on either a Dark Reader transilluminator or a 312 nm UV device and the lowest detectable amount of fluorescein recorded. The actual fluorescein volumes used were: nitrocellulose membrane - 0.5 uL; horizontal gel bed - 2 uL; vertical gel glass plate - 2 uL; 96-well plate - 100 uL; centrifuge tube - 400 uL.
    Clare Chemical Research

    FAQ
    User Feedback
    Dark Reader Lit.
    Journal Refs.
    Links
    SiteMap
    No UV
    No DNA Damage
    Improve Cloning
    Cut Bleaching
    See through Walls
    Contact
    Transilluminators
    Lamps
    GelHead
    Accessories
    Home
    Theory
    Ordering
    GelGreen
    SYBR Gold
    SYBR Green
    SYBR Safe
    GelStar
    Ethidium
    GFPs
    Transgenics
    fluorescein
    Pro-Q Diamond
    SYPRO Ruby
    SYPRO Orange
    How it Works
    Which Dyes?
    Products
    Support
    Examples